Product Information

As a naturally occurring flavonoid compound, spinosad has shown great potential for application in fields such as medicine and food. In terms of medicine, stylosanthin has significant anti-tumor activity, which can effectively inhibit the proliferation of breast cancer, prostate cancer and colon cancer cells, providing new possibilities for cancer treatment. Its excellent antioxidant properties help to eliminate excessive free radicals in the body, reduce oxidative stress damage to cells, and thus have important implications for the prevention and treatment of cardiovascular diseases, neurodegenerative diseases, and other conditions. Spiny stem flower extract also has various pharmacological effects such as regulating blood lipids, antibacterial, anti-inflammatory, etc., playing a multidimensional positive role in maintaining human health.

In the field of food, thorn stem flower extract, due to its natural antioxidant properties, can be used as a food preservative, effectively extending the shelf life of food, preventing food oxidation and spoilage, and maintaining the nutritional content and flavor of food. Its phytoestrogenic properties have attracted much attention in the development of functional foods, such as being used to develop health foods for women and help alleviate menopausal symptoms.

With the continuous increase in demand for spinocarpin, in-depth exploration of its production process and detection methods has become particularly urgent. Efficient production processes can increase the yield and quality of spinocarpin, reduce production costs, and meet the growing market demand for it. Accurate and reliable detection methods are the key to ensuring the quality of prickly stem flower extract products, which can guarantee their safe and effective application in fields such as medicine and food. Therefore, in-depth research on the production process and detection methods of thorn stem anthocyanins has extremely important practical significance and application value.

Production process of  formononetin

（1） Extraction process

Raw material selection: Spiny stem flower extract is widely present in various plants, such as leguminous plants such as Changchun oil hemp vine and soybeans. There are differences in the content of anthocyanins in different raw materials, which makes them have their own characteristics in extraction processes and applications. Changchun oil hemp vine, as a common medicinal plant, contains a certain amount of spinocarpin in its stem. Research has shown that the content of thorn stem anthocyanins in Changchun oil hemp vine is relatively stable and easy to collect and process. Soybeans, as an important crop, also contain spinosad in their seeds. Soybeans are abundant in resources, widely planted, and widely used in the food industry and other fields. Extracting spinocarpin from soybeans has important economic value and application prospects.

Traditional extraction method: The traditional extraction method mainly uses solvent extraction method, using methanol as the solvent, to extract spinocarpin from Changchun oil hemp vine. During the extraction process, after crushing the Changchun oil hemp vine, methanol was added according to a certain ratio of material to liquid, and refluxed and extracted three times at 85 ℃ for 1.5 hours each time. Through this method, it is possible to effectively dissolve the extract of spinocarpin from plant tissues, and then obtain an extract containing spinocarpin through steps such as filtration and concentration. Although this method is relatively simple to operate, it has disadvantages such as low extraction efficiency and high solvent consumption.

（2） Synthesis process

Raw materials and principles: The synthesis process of thorn stem flower extract mainly uses p-methoxyphenylacetic acid and meta phenylbisphenol as raw materials, and is achieved through a series of chemical reactions. Its synthesis principle is based on condensation reactions and cyclization reactions in organic chemistry. In the condensation reaction, methoxyphenylacetic acid and meta phenylbisphenol undergo intermolecular condensation under the action of a specific catalyst, forming an intermediate with a certain structure. Then, through the cyclization reaction, the intermediate undergoes further intramolecular cyclization, ultimately forming the molecular structure of spinocarpin. The key to this synthesis method lies in selecting appropriate catalysts and reaction conditions to ensure the smooth progress of the reaction and high purity of the product.

Specific synthesis steps: Firstly, add p-methoxyphenylacetic acid and meta phenylbisphenol in a certain proportion to the reaction vessel, then add an appropriate amount of boron trifluoride tetrahydrofuran solution as a catalyst, control the reaction temperature at 40-50 ℃, stir the reaction for 3-5 hours, and allow the raw materials to undergo sufficient condensation reaction. Next, cool the reaction solution to room temperature, add an appropriate amount of water, and continue stirring the reaction for 5-10 hours to allow the reaction to proceed further. Then stop stirring and filter to obtain the solid substance generated by the reaction. Recrystallize the obtained solid material using a certain proportion of water methanol mixed solvent, filter again, and obtain the intermediate with high purity. Mix the intermediate with boron trifluoride tetrahydrofuran solution and slowly add N, N-dimethylformamide dropwise at 13 ℃ to form solution A. In another container, add phosphorus oxychloride dropwise to N, N-dimethylformamide. After the dropwise addition is complete, raise the temperature to 55 ℃ and react for 20 minutes to obtain solution B. Cool solution A to below 5 ℃ with ice water, then slowly add solution B dropwise. Strictly control the temperature below 20 ℃ during the dropwise addition process. After the dropwise addition is complete, continue the reaction for 3 hours. When the test results show that the raw material content is less than 5%, add the reaction solution dropwise to a 37% hydrochloric acid solution at 85 ℃, reflux for 1 hour, and then cool, filter, and wash the precipitated solid with water to obtain the crude product. Finally, the crude product can be recrystallized using a water methanol mixed solvent to obtain high-purity spinocarpin. Throughout the entire synthesis process, it is necessary to strictly control the conditions of each reaction step, including temperature, time, reagent dosage, etc., to ensure the smooth progress of the reaction and the quality of the product.

Technological advantages: Compared with traditional extraction methods, this synthesis process has many advantages. In terms of safety, the boron trifluoride tetrahydrofuran solvent used has a higher boiling point and lower volatility compared to traditional solvents such as ether, reducing safety risks such as flammability and explosiveness. In terms of cost, production costs have been reduced by optimizing reaction conditions and using relatively inexpensive raw materials. Moreover, the operation of this synthesis process is relatively simple and does not require complex equipment and technology, which is conducive to the promotion of industrial production. Through continuous optimization of the synthesis process, the synthesis efficiency and quality of spinocarpin have been significantly improved, providing strong support for its large-scale production and application.

As a natural compound with broad application prospects, the research on its production process and detection methods is crucial. In terms of production technology, there are various choices of raw materials in the extraction process. Although the traditional solvent extraction method is simple to operate, it has shortcomings. The application of new extraction devices and technologies such as ultrasound assisted extraction technology has significantly improved the extraction efficiency. The synthesis process uses p-methoxyphenylacetic acid and meta phenylbisphenol as raw materials, and through specific reaction steps and optimized reaction conditions, it has the advantages of high safety, low cost, and easy operation, providing the possibility for large-scale production.